Coding
Part:BBa_K1123016:Design
Designed by: Ardjan van der Linden Group: iGEM13_TU-Eindhoven (2013-08-31)
DNA Coding Sequence for 1G70 Protein
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 64
Illegal NgoMIV site found at 148
Illegal NgoMIV site found at 232
Illegal NgoMIV site found at 316 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The protein sequence was codon optimized for expression in E.coli bacteria. We then repeated the sequence a number of times to amplify the protein. These repeats were held together using small linkage sequences of Glycine and Serine amino acids. In total the 1G70 protein sequence was repeated 5 times.
Source
The amino acid sequence for this parts was retrieved from PDB.org after a database query was performed looking for proteins with high Arginine and Lysine contents