Coding

Part:BBa_K1123016:Design

Designed by: Ardjan van der Linden   Group: iGEM13_TU-Eindhoven   (2013-08-31)


DNA Coding Sequence for 1G70 Protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 64
    Illegal NgoMIV site found at 148
    Illegal NgoMIV site found at 232
    Illegal NgoMIV site found at 316
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The protein sequence was codon optimized for expression in E.coli bacteria. We then repeated the sequence a number of times to amplify the protein. These repeats were held together using small linkage sequences of Glycine and Serine amino acids. In total the 1G70 protein sequence was repeated 5 times.


Source

The amino acid sequence for this parts was retrieved from PDB.org after a database query was performed looking for proteins with high Arginine and Lysine contents

References